Our group investigates the functional mechanism of membrane-embedded heterooligomeric macromolecular machines in their native environments. We characterize the structural transitions, conformational equilibria, and the thermodynamic parameters to understand the mechanistic basis of their function. To achieve this goal, we employ a highly interdisciplinary approach involving recombinant membrane protein expression and purification, biochemical/biophysical characterization, and electron spin resonance spectroscopy (ESR) techniques combined with structural modeling.
The group focuses on four main aspects:
Development and application of new approaches for in-situ electron spin resonance spectroscopy of membrane protein complexes
Membrane transport mechanism of ABC transporters and symporters
Protein folding and insertion by the β-Barrel Assembly Machinery (BAM) complex
Lipopolysaccharide (LPS) transport by the Lpt system
Our group is located at the Institute of Biophysics in the Department of Physics. We are associated with the Center for Biomolecular Magnetic Resonance (BMRZ) . Our research is funded by the Emmy Noether-Program of the Deutsche Forschungsgemeinschaft (DFG) and the Collaborative Research Center, CRC 807.
Welcome to Marina Dajka! She starts her PhD in our group.
Welcome to Aathira Gopinath! She starts her PhD in our group.
Welcome to Michael Rudolph! He starts his PhD in our group.
Our collaborative work with the Geertsma and the Hummer group titled 'Structural basis for functional interactions in dimers of SLC26 transporters' is accepted for publication in Nature Communications!
Welcome to Sophie Ketter! She starts her Bachelor Thesis in the group.
Our manuscript titled 'In-situ observation of conformational dynamics and protein- ligand/substrate interaction in outer membrane proteins with DEER/PELDOR spectroscopy' is accepted for publication in Nature Protocols!
Our new group is all set to start from 1st Jan 2019. Plenty of lab and office space waiting!
Benesh became the member of the Johanna Quandt Young Academy at Goethe (JQYA)
Benesh receives the Young Investigator Award of the German Biophysical Society!
The DFG Senate approves our Emmy Noether Research Group! Exciting time ahead...